Data/hora: 20/05/2024 14:05:29

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 09/09/1993
Data da última atualização: | 09/09/1993
Autoria: | TSAI, M.; SU, H.
Afiliação: | National Taiwan University, Dept. of Plant Pathology and Entomology, Taipei, Taiwan, People's Republic of China.
Título: | Development and characterization of monoclonal antibodies to citrus tristeza virus (CTV) strains in Taiwan.
Ano de publicação: | 1991
Fonte/Imprenta: | Riverside, California: International Organization of Citrus Virologists, 1991.
Páginas: | p.46-50
Idioma: | Inglês
Notas: | Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside California, Proceedings...
Conteúdo: | Twelve stable hybridoma cell lines secreting CTV-specific antibodis were obtained against purified sources of SY-M, a mild seedling-yellows strain of CTV, and CTV-D, a non-seedling-yellows dwarfing strain. These monoclonal antibodies (MAB) included IgG 1, IgG 2a, and IgM subtypes. Immunuglobulin titers up to 4,096 X and 83,886,080 X were obtained in culture supernatants and ascites fluid respectively. To MABs were found which reacted differently to mild and severe CIV strains in double antibody sandwich-indirect ELISA. Thirtheen domestic and foreing isolates of CTV showed six distinct reaction patterns when tested with the two MABs from Taiwan plus MABMCA13 from Florida and MAB 3DF1 from Spain The MAB 4H6-1 is distinct from previously described MAB. The MAB 10E3-4 reacts similary to MCA13, but shows at least some quantitative differences with several isolates.
Palavras-Chave: | Pummelo; Stem pitting.
Thesagro: | Elisa.
Thesaurus Nal: | serotypes.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13272-1) - ADC - PL - -- - 634.3040601 - C748p - 1992000183 - DIS